Print this page Email this page | Users Online: 307
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2022  |  Volume : 13  |  Issue : 2  |  Page : 49-53

Comparative analysis of staining efficacy of Leishman's stain with hematoxylin and eosin stain in the assessment of keratin pearl formation and mitotic figures in different grades of oral squamous cell carcinoma


Unit of Oral Pathology and Microbiology, Faculty of Dental Sciences, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

Correspondence Address:
Dr. Rahul Agrawal
Unit of Oral Pathology and Microbiology, Faculty of Dental Sciences, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/srmjrds.srmjrds_49_22

Rights and Permissions

Background: Keratin is an intermediate protein that structures the cytoskeleton of all epithelial cells. Keratin pearl is present in various pathological conditions such as oral squamous cell carcinoma (OSCC). Mitotic figures can be used as a valuable tool in assessing cellular proliferation and act as a prognostic indicator in dysplasia and malignancy of the oral cavity. Aim: The aim of this study was to compare the efficacy of Leishman's stain with hematoxylin and eosin (H and E) stain in assessing keratin pearls and mitotic figures. Materials and Methods: In total, 30 diagnosed cases of OSCC of three different histological grades were taken. In each case, two sections of 4–5-micron thickness were made and stained with routine H and E stain and Leishman's stain. Staining intensity and specificity results were analyzed statistically using the Chi-square test and P = 0.05 was considered to be statistically significant. Results: Both stains can distinguish three different types of OSCC histologically. Leishman's stain demonstrated staining intensity (P = 0.087) and specificity (P = 0.017) comparable to that of routine H and E. Conclusion: Leishman's stain distinctly stained the keratin pearl and mitotic figures with a higher intensity. However, H and E showed a higher specificity.


[FULL TEXT] [PDF]*
Print this article     Email this article
Next article
Previous article
Table of Contents

Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Citation Manager
Access Statistics
Reader Comments
Email Alert*
Add to My List*
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed846    
    Printed44    
    Emailed0    
    PDF Downloaded122    
    Comments [Add]    

Recommend this journal