Print this page Email this page | Users Online: 361
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 

 Table of Contents  
Year : 2020  |  Volume : 11  |  Issue : 3  |  Page : 138-141

Analysis of lip prints as a predictive tool in screening Type 2 diabetes mellitus – A cross-sectional study

Department of Oral Medicine and Radiology, Mahatma Gandhi Postgraduate Institute of Dental Sciences, Puducherry, India

Date of Submission25-Apr-2020
Date of Acceptance20-Aug-2020
Date of Web Publication15-Oct-2020

Correspondence Address:
Dr. Vineeth Ramanathan
Department of Oral Medicine and Radiology, Mahatma Gandhi Postgraduate Institute of Dental Sciences, Puducherry
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/srmjrds.srmjrds_30_20

Rights and Permissions

Background: Globally, the prevalence of diabetes is soaring among middle- and low-income countries. Lip print is a noninvasive procedure which is being genetically determined to serve as a tool in forensic dentistry. Hence, the present study was conducted to assess the role of lip prints among Type 2 diabetes mellitus (DM). Aims and Objectives: The aim and objective of the study were to assess the lip pattern of the individual among diabetic and nondiabetic individuals, and the objective was to compare and correlate lip print pattern in patients with Type 2 DM. Materials and Methods: The study was conducted on 25 uncontrolled Type 2 DM patients and 25 healthy controls. Lip prints were obtained and classified using Suzuki and Tsuchihashi's classification. Statistical Analysis: The software SPSS (Statistical Package for the Social Sciences) version 20 was used for statistical analysis. Chi-square test was used for comparison. Results: The results of the present study demonstrated that Type 4 reticular pattern was significantly higher among diabetic patients. Conclusion: The present study showed that lip prints may serve as a predictive tool in screening Type 2 DM.

Keywords: Diabetes mellitus, lip prints, reticular pattern

How to cite this article:
Ramanathan V, Kumaran JV, Srinivasan SV, Daniel MJ. Analysis of lip prints as a predictive tool in screening Type 2 diabetes mellitus – A cross-sectional study. SRM J Res Dent Sci 2020;11:138-41

How to cite this URL:
Ramanathan V, Kumaran JV, Srinivasan SV, Daniel MJ. Analysis of lip prints as a predictive tool in screening Type 2 diabetes mellitus – A cross-sectional study. SRM J Res Dent Sci [serial online] 2020 [cited 2023 Feb 1];11:138-41. Available from:

  Introduction Top

Diabetes mellitus (DM) is a metabolic disorder characterized by a common feature of chronic hyperglycemia with disturbance of carbohydrate, protein, and fat metabolism (WHO 2018). There is an immense rise in the diabetic people from 108 million in 1980 to 422 million in 2014. Normal lines and fissures in the form of wrinkles and grooves present in the zone of transition of human lip between the inner labial mucosa and outer skin are called lip prints, and examination of which is known as cheiloscopy.[1] “Cheilos” is derived from Greek word meaning lip and “scopy” meaning to examine.[2] In 1902, R. Fischer, an anthropologist was the first to describe the biological phenomenon of systems of furrows on the red part of human lips.[3] Type 2 DM is a complex disorder, and it is influenced by genetic factors. The biochemical level of blood or urine is assessed for glucose estimation in an individual. Many patients with diabetes are left undiagnosed and lead to many serious complications such as coronary artery disease, stroke, and peripheral vascular disease. Recently, the increased prevalence of diabetes in the medical field leads to an interest in search of potential early biomarkers. The present study was conducted to assess the association of lip prints in diabetic and nondiabetic patients among the South Indian population. In our present study, analysis of lip print patterns was done with a magnifying lens and image scanner. The objective of the present study was to ascertain whether there is an association of lip print patterns with diabetics and to compare lip print patterns of diabetic and nondiabetic individuals.

  Materials and Methods Top

Study samples

The materials used for the lip print pattern analysis were as shown in [Figure 1]. Blood samples were collected from the Outpatient Department of Oral Medicine and Radiology, at Mahatma Gandhi Post Graduate Institute of Dental Sciences, Pondicherry, and confirmed by clinical and laboratory assessment of the last three consecutive fasting blood sugar values >140 mg/100 ml was set as the criteria to proffer the uncontrolled state of the disease. Individuals with age limit between 35 and 55 years (mean age = 45) who are willing to participate and for those diabetes are confirmed by laboratory assessment were included in Group II. Individuals with three consecutive fasting blood sugar values between 126 and 140 mg/100 ml were included in Group I. Individuals with genetic disease, other systemic diseases, inflammation, trauma, malformation, deformity, surgical scars, ulcers, burns, and other abnormalities of the lips were excluded from both the groups.
Figure 1: Photograph of materials used in the study

Click here to view

Method of collection of data


The procedure was clearly explained to the participants. The data were collected by utilizing a structured questionnaire that includes the demographic data such as name, age, gender, marital status, place, and education. An informed consent was obtained from the subjects, and they were categorized in the following groups.

  • Group I: 25 normal individuals without Type 2 DM
  • Group II: 25 individuals with Type 2 DM.

Lip print procedure and analysis

The lips were cleaned thoroughly with tissue paper to avoid contamination, and then lipstick was taken on one end of an ear bud applied gently starting at the midline and moving laterally in the upper lip. The same technique was repeated for the lower lip. The participants were asked to gently rub his/her lips for its uniform application. The individuals were asked to retain a relaxed lip position, and the impression was taken on the glued portion of the cellophane tape. Lip prints were obtained by dabbing the tape in the center first and then pressing it comfortably toward the corner of the lips [Figure 2]. The tape was carefully lifted from one end of the lip to avoid any smudging of the print.[4] The impression was then stuck on plain A4 paper [Figure 3]. Sterilized cotton and Vaseline were used to clean the lips properly. After acquiring the patterns of the individuals, each of them was assigned a definite number and studied.
Figure 2: Photograph of application of glued portion of cellophane tape

Click here to view
Figure 3: Photograph of lipstick print

Click here to view

The lip prints were divided into four quadrants and recorded as:

  • Right upper lip quadrant RU
  • Right lower quadrant RL
  • Left upper quadrant LU
  • Left lower quadrant LL


The slides were studied carefully with a magnifying lens.[5],[6] The lip prints of each individual were scanned using an image scanner. They were stored as JPEG (Joint Photographic Experts Group) files for maximum details to analyse the lip patterns [7] denoting the type according to Suzuki and Tsuchihashi's (1970) classification. Only 10 mm area from the center portion of the upper lip was considered to avoid any errors from overlapping of lip prints on the right and left sides of the lip.[8]

Statistical analysis

The software SPSS version 20 (Statistical package for the social sciences) was used for statistical analysis. Chi-square test was used for comparison.

Suzuki and Tsuchihashi's lip print pattern [Figure 4]
Figure 4: Suzuki and Tsuchihashi's lip print pattern

Click here to view

  • Type I: complete straight grooves
  • Type I': partial straight grooves
  • Type II: branched grooves
  • Type III: intersected grooves
  • Type IV: reticular grooves
  • Type V: undifferentiated grooves.

  Results Top

In our study, of 50 study participants (Groups 1 and 2), reticular type of lip print pattern was significantly higher (52%) than controls.

Lip print pattern

The pattern of lip print in cases and controls was classified into Types I, I', II, III, IV, and V. The percent distribution of each lip print pattern in cases was 12% Type I, nil cases in Type I' and III, 32% Type II, 52% Type IV, and 4% Type V patterns. The percentage distribution of lip print patterns in controls was 32% Type I, 8% Type I', 24% Type II, 24% Type III, 4% Type IV, and 8% Type V patterns.

[Table 1] shows lip print patterns among cases and controls. The difference in lip print patterns between cases and controls was statistically highly significant (P ≤ 0.001). The difference was also compared in both sexes [Table 2].
Table 1: Distribution of cases and controls based on the types of lip prints

Click here to view
Table 2: Distribution of lip prints based on gender

Click here to view

  Discussion Top

India represents the world's diabetes burden, with an estimated 72 million cases in 2017, expected to double to 134 million by 2025. Diabetic prevalence in the midst of adults over 18 years of age has increased from 4.7% in 1980 to 8.5% in 2014 (WHO 2018). The major causes of diabetes are blindness, kidney failure, heart attacks, stroke, and lower limb amputation. It is estimated that 1.6 million deaths were directly caused by diabetes in the year 2016. Nearly 50% of all deaths caused due to high blood glucose occur before the age of 70 years. Diabetes was the seventh leading cause of death in 2016, stated by the World Health Organization.

The result shows that the people with a reticular type of lip pattern are common among Type 2 DM. The distribution of the cases and controls based on gender and types of lip prints showed in [Graph 1]. Reticular pattern was higher in cases among males (50%) and females (53.8%), followed by Type I 8.3% and 15.4%, Type II 33.3% and 30.8%, and Type IV 8.3% and nil cases, respectively. Complete straight, branched, and intersected pattern was higher in controls among males with equal distribution of 33.3% each. Reticular pattern was least among females with 4.5%. Thus, people with reticular lip print patterns may have a greater chance of developing Type 2 DM.

The present study showed that 52% of cases were Type IV (reticular) pattern, and in controls, the highest percentage was found for Type I (32%), further Type IV (4%) was the least pattern type in controls. Reticular type pattern was higher in cases than controls. The observation of the present study is in correlation with the study conducted by ManjushaandSudha [3] who reported the percentage distribution of each lip print pattern in cases as 57% Type IV, followed by 20% Type III, 8% Type I, 6% Type I', 4% Type II, and 5% Type V patterns.

Manjusha andSudha [3] in their study found that 63.0% of female patients had reticular pattern compared with female controls (20.5%). Branched and vertical pattern was prevalent among female controls than cases. This study was similar to the study by Umana et al. who found that reticular and undifferentiated patterns had chances of developing diabetes compared with branched and intersected pattern.

According to the Indian Council of Medical Research, the North Indian population is less likely to develop DM than the South Indian population. Arora et al. suggested that this difference could be due to the fact that the North Indians are migrant Asian populations, and South Indians are the host populations.[9] The people of the Malabar region with reticular pattern are at high risk of developing diabetes, whereas people with branched pattern have less chances to develop the disease.[3]

Lip prints with reticular pattern may serve as a potential risk factor in assessing the Type 2 DM. Long-term follow-up studies to be carried out among different parts of the country to further validate these results. Therefore, to conclude, the individuals with reticular type of lip pattern are more likely to develop DM.


It includes a small sample size, since diabetes is influenced by environmental and genetic factors, it is necessary to confirm the present study from various areas of the country and globally. Recommendations are put forth to study the lip prints of the offspring to predict the presence of diabetes, especially those with a family history, to further validate the findings of this study.

  Conclusion Top

Our results showed that Type IV reticular pattern of lip print was significantly more in diabetic than nondiabetics, thus suggesting that this pattern has a greater predilection of developing DM, which can be used as a predictive tool in mass screening of DM.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

  References Top

Sathwane R. Correlation of lip and finger prints in patients with type 2 diabetes mellitus. Int J Curr Res 2019;11:1630-3.  Back to cited text no. 1
Mhaske SP, Jagtap K, Deshpande R. Cheiloscopy tool for forensic identification. IJDPMS 2013;1 :24-32.  Back to cited text no. 2
Manjusha P, Sudha S. Analysis of lip print and finger print patterns in patients with type 2 diabetes mellitus: J Oralmaxillofac Pathology 2017;21:309-15.  Back to cited text no. 3
Aparna B. Mujib A. Evaluation and comparison of various methods of lip prints in gender determination and its stability over a period of time. Int J Anat Res 2018;6: 5167-71.  Back to cited text no. 4
Umana UE. Dermatoglyphic and cheiloscopic patterns among diabetic patients: A study in Ahmadu Bello University Teaching Hospital Zaria. Nigeria J Biol Life Sci 2013;4:206-14.  Back to cited text no. 5
Sultana Q, Shariff MH. Cheiloscopy: A scientific approach for personal identification. Int J Anat Res 2014;2:668-72.  Back to cited text no. 6
Jatti D, Rastogi P. Digital Analysis of Lip Prints for Personal Identification: A Cross Sectional Study in South Indian Populatio J Indian Acad Forensic Med 2015;37:3.  Back to cited text no. 7
Ranjan V, Sunil MK, Kumar R. Study of lip prints: A forensic study Journal of Indian Academy of Oral Medicine and Radiology 2014;26.  Back to cited text no. 8
Arora V, Malik JS, Khanna P, Goyal N, Kumar N, Singh M. Prevalence of diabetes in urban Haryana. Australia Med J 2010;3:488 94.  Back to cited text no. 9


  [Figure 1], [Figure 2], [Figure 3], [Figure 4]

  [Table 1], [Table 2]

This article has been cited by
1 Dermatoglyphics and Cheiloscopy patterns in hypertensive and type 2 Diabetes mellitus patients: An observational study
CD Mouneshkumar,Santosh Anand,RH Shilpa,Nazia Haidry,Pranay Kulkarni,Pranay Aastha,Aaysha Gupta
Journal of Family Medicine and Primary Care. 2021; 10(3): 1177
[Pubmed] | [DOI]


Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

  In this article
Materials and Me...
Article Figures
Article Tables

 Article Access Statistics
    PDF Downloaded166    
    Comments [Add]    
    Cited by others 1    

Recommend this journal